› 1q29

X-ray diffraction
3Å resolution

Hammerhead Ribozyme with 5'-5' G-G linkage: Conformational change experiment

Released:
DOI: 10.2210/pdb1q29/pdb
PDB entry 1q29 coloured by chain, front view.
PDB entry 1q29 coloured by chain, side view.
PDB entry 1q29 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
A helical twist-induced conformational switch activates cleavage in the hammerhead ribozyme.
Dunham CM, Murray JB, Scott WG
J Mol Biol 332 327-36 (2003)
PMID: 12948485

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196166 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*GP*UP*GP*GP*UP*CP*UP*GP*AP*UP*GP*AP*GP*GP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 17 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Synthetic construct
Expression system: Not provided
Ribozyme Chain: B
Molecule details ›
Chain: B
Length: 24 nucleotides
Theoretical weight: 7.67 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CO 5 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 66.689Å b: 66.689Å c: 140.528Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.242 0.279
Expression system: Not provided

Quick links

Citations

7 review citations

RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.
Šponer et al. (2018)
6 more

4 mentions without citation

Describing RNA structure by libraries of clustered nucleotide doublets.
Sykes et al. (2005)
3 more