› 1q34

X-ray diffraction
2.9Å resolution

Crystal structures of two UBC (E2) enzymes of the ubiquitin-conjugating system in Caenorhabditis elegans

Released:
DOI: 10.2210/pdb1q34/pdb
PDB entry 1q34 coloured by chain, front view.
PDB entry 1q34 coloured by chain, side view.
PDB entry 1q34 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Entry authors: Schormann N, Lin G, Li S, Symersky J, Qiu S, Finley J, Luo D, Stanton A, Carson M, Luo M, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 163 amino acids
Theoretical weight: 18.61 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52478 (Residues: 1-163; Coverage: 85%)
Gene names: C35B1.1, ubc-1
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 100.922Å b: 37.584Å c: 103.881Å
α: 90° β: 117.58° γ: 90°
R-values:
R R work R free
0.239 0.233 0.266
Expression system: Escherichia coli BL21(DE3)

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