› 1q3e

X-ray diffraction
1.9Å resolution

HCN2J 443-645 in the presence of cGMP

Released:
DOI: 10.2210/pdb1q3e/pdb
PDB entry 1q3e coloured by chain, front view.
PDB entry 1q3e coloured by chain, side view.
PDB entry 1q3e coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for modulation and agonist specificity of HCN pacemaker channels.
Zagotta WN, Olivier NB, Black KD, Young EC, Olson R, Gouaux E
Nature 425 200-5 (2003)
PMID: 12968185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-131576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88703 (Residues: 443-645; Coverage: 24%)
Gene names: Bcng2, Hac1, Hcn2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PCG 2 x PCG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I4
Unit cell:
a: 94.236Å b: 94.236Å c: 124.008Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

68 review citations

Hyperpolarization-activated cation channels: from genes to function.
Biel et al. (2009)
67 more

17 mentions without citation

A structural view of ligand-dependent activation in thermoTRP channels.
Steinberg et al. (2014)
16 more