› 1q3g

X-ray diffraction
2.65Å resolution

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L, W, X, Y, Z
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, W, X, Y, Z
Length: 419 amino acids
Theoretical weight: 44.79 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33038 (Residues: 1-419; Coverage: 100%)
Gene names: ECL_04571, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand UDA 16 x UDA
Ligand EDO 16 x EDO
1 modified residue:
Ligand IAS 16 x IAS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 139.459Å b: 153.934Å c: 167.481Å
α: 90° β: 112.95° γ: 90°
R-values:
R R work R free
0.217 0.217 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
10 more

2 mentions without citation

Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Zhu et al. (2012)
1 more