› 1q4u

X-ray diffraction
1.6Å resolution

Crystal structure of 4-hydroxybenzoyl CoA thioesterase from arthrobacter sp. strain SU complexed with 4-hydroxybenzyl CoA

Released:
DOI: 10.2210/pdb1q4u/pdb
PDB entry 1q4u coloured by chain, front view.
PDB entry 1q4u coloured by chain, side view.
PDB entry 1q4u coloured by chain, top view.
Source organism: Arthrobacter sp. SU
Primary publication:
The structure of 4-hydroxybenzoyl-CoA thioesterase from arthrobacter sp. strain SU.
Thoden JB, Zhuang Z, Dunaway-Mariano D, Holden HM
J Biol Chem 278 43709-16 (2003)
PMID: 12907670

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoyl-CoA + H(2)O = 4-hydroxybenzoate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxybenzoyl-CoA thioesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 16.42 KDa
Source organism: Arthrobacter sp. SU
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04416 (Residues: 1-151; Coverage: 100%)
Gene names: fcbC, fcbC1
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments


Cofactor: Ligand 4CA 2 x 4CA
1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 112.9Å b: 112.9Å c: 60.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.213
Expression system: Escherichia coli

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Citations

1 review citation

Biotechnological potential of the ethylmalonyl-CoA pathway.
Alber BE. (2011)
39 other articles

6 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
5 more