› 1q7m

X-ray diffraction
2.1Å resolution

Cobalamin-dependent methionine synthase (MetH) from Thermotoga maritima (Oxidized, Monoclinic)

Released:
DOI: 10.2210/pdb1q7m/pdb
PDB entry 1q7m coloured by chain, front view.
PDB entry 1q7m coloured by chain, side view.
PDB entry 1q7m coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structures of the N-terminal modules imply large domain motions during catalysis by methionine synthase.
Evans JC, Huddler DP, Hilgers MT, Romanchuk G, Matthews RG, Ludwig ML
Proc Natl Acad Sci U S A 101 3729-36 (2004)
PMID: 14752199

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 566 amino acids
Theoretical weight: 63.63 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WYA5 (Residues: 1-566; Coverage: 74%)
Gene name: TM_0268
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 59.7Å b: 84.48Å c: 125.67Å
α: 90° β: 100.87° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
12 more

4 mentions without citation

Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily.
Chatterjee et al. (2008)
3 more