› 1q7r

X-ray diffraction
1.9Å resolution

X-ray crystallographic analysis of a predicted amidotransferase from B. stearothermophilus at 1.9 A resolution

Released:
DOI: 10.2210/pdb1q7r/pdb
PDB entry 1q7r coloured by chain, front view.
PDB entry 1q7r coloured by chain, side view.
PDB entry 1q7r coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Miller DJ, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reactions catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PdxT Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.44 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83813 (Residues: 1-196; Coverage: 100%)
Gene name: pdxT
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 1 x CL
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P43212
Unit cell:
a: 44.947Å b: 44.947Å c: 202.668Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.232
Expression system: Escherichia coli BL21(DE3)

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