› 1q7t

X-ray diffraction
1.9Å resolution

Rv1170 (MshB) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1q7t/pdb
PDB entry 1q7t coloured by chain, front view.
PDB entry 1q7t coloured by chain, side view.
PDB entry 1q7t coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of MshB from Mycobacterium tuberculosis, a deacetylase involved in mycothiol biosynthesis.
McCarthy AA, Peterson NA, Knijff R, Baker EN
J Mol Biol 335 1131-41 (2004)
PMID: 14698305

Function and Biology Details

Reaction catalysed: 
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H(2)O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 34.88 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJN3 (Residues: 1-303; Coverage: 100%)
Gene names: Rv1170, mshB
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like

Ligands and Environments

2 bound ligands:
Ligand BOG 3 x BOG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 71.69Å b: 83.74Å c: 95.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.215
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
11 more

6 mentions without citation

Structure and function of the LmbE-like superfamily.
Viars et al. (2014)
5 more