› 1q8i

X-ray diffraction
2Å resolution

Crystal structure of ESCHERICHIA coli DNA Polymerase II

Released:
DOI: 10.2210/pdb1q8i/pdb
PDB entry 1q8i coloured by chain, front view.
PDB entry 1q8i coloured by chain, side view.
PDB entry 1q8i coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Brunzelle JS, Muchmore CRA, Mashhoon N, Blair-Johnson M, Shuvalova L, Goodman MF, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase II Chain: A
Molecule details ›
Chain: A
Length: 783 amino acids
Theoretical weight: 90.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21189 (Residues: 1-783; Coverage: 100%)
Gene names: JW0059, b0060, dinA, polB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B, APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 92.383Å b: 116.596Å c: 82.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.236
Expression system: Escherichia coli

Quick links