› 1q95

X-ray diffraction
2.46Å resolution

Aspartate Transcarbamylase (ATCase) of Escherichia coli: A New Crystalline R State Bound to PALA, or to Product Analogues Phosphate and Citrate

Released:
DOI: 10.2210/pdb1q95/pdb
PDB entry 1q95 coloured by chain, front view.
PDB entry 1q95 coloured by chain, side view.
PDB entry 1q95 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Aspartate transcarbamylase (ATCase) of Escherichia coli: a new crystalline R-state bound to PALA, or to product analogues citrate and phosphate.
Huang J, Lipscomb WN
Biochemistry 43 6415-21 (2004)
PMID: 15157075

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141554 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate carbamoyltransferase catalytic subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 310 amino acids
Theoretical weight: 34.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A786 (Residues: 2-311; Coverage: 100%)
Gene names: JW4204, b4245, pyrB
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase
Aspartate carbamoyltransferase regulatory chain Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 153 amino acids
Theoretical weight: 17.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7F3 (Residues: 1-153; Coverage: 100%)
Gene names: JW4203, b4244, pyrI
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PAL 6 x PAL
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 125.51Å b: 153.49Å c: 185.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.23 0.27
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Disulfides as redox switches: from molecular mechanisms to functional significance.
Wouters et al. (2010)
1 more

4 mentions without citation

Allostery and cooperativity in Escherichia coli aspartate transcarbamoylase.
Kantrowitz ER. (2012)
3 more