› 1qaj

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURES OF THE N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE COMPLEXED WITH NUCLEIC ACID: FUNCTIONAL IMPLICATIONS FOR TEMPLATE-PRIMER BINDING TO THE FINGERS DOMAIN

Released:
DOI: 10.2210/pdb1qaj/pdb
PDB entry 1qaj coloured by chain, front view.
PDB entry 1qaj coloured by chain, side view.
PDB entry 1qaj coloured by chain, top view.
Source organism: Moloney murine leukemia virus
Primary publication:
Crystal structures of an N-terminal fragment from Moloney murine leukemia virus reverse transcriptase complexed with nucleic acid: functional implications for template-primer binding to the fingers domain.
Najmudin S, Coté ML, Sun D, Yohannan S, Montano SP, Gu J, Georgiadis MM
J Mol Biol 296 613-32 (2000)
PMID: 10669612

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114426 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Reverse transcriptase/ribonuclease H Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 29.35 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 683-937; Coverage: 15%)
Gene name: gag-pol
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
DNA (5'-D(*CP*AP*TP*GP*CP*AP*TP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 65.868Å b: 63.587Å c: 73.398Å
α: 90° β: 102.91° γ: 90°
R-values:
R R work R free
0.2 0.2 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

A structural basis for processivity.
Breyer et al. (2001)
2 more