› 1qao

X-ray diffraction
2.7Å resolution

THE STRUCTURE OF THE RRNA METHYLTRANSFERASE ERMC': IMPLICATIONS FOR THE REACTION MECHANISM

Released:
DOI: 10.2210/pdb1qao/pdb
PDB entry 1qao coloured by chain, front view.
PDB entry 1qao coloured by chain, side view.
PDB entry 1qao coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The 2.2 A structure of the rRNA methyltransferase ErmC' and its complexes with cofactor and cofactor analogs: implications for the reaction mechanism.
Schluckebier G, Zhong P, Stewart KD, Kavanaugh TJ, Abad-Zapatero C
J Mol Biol 289 277-91 (1999)
PMID: 10366505

Function and Biology Details

Reaction catalysed: 
2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA adenine N-6-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.96 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P13956 (Residues: 1-244; Coverage: 100%)
Gene name: ermC'
Sequence domains: Ribosomal RNA adenine dimethylase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 81.46Å b: 81.46Å c: 121.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.281
Expression system: Escherichia coli

Quick links

Citations

4 review citations

AdoMet-dependent methylation, DNA methyltransferases and base flipping.
Cheng et al. (2001)
3 more

16 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
15 more