› 1qba

X-ray diffraction
1.85Å resolution

BACTERIAL CHITOBIASE, GLYCOSYL HYDROLASE FAMILY 20

Released:
DOI: 10.2210/pdb1qba/pdb
PDB entry 1qba coloured by chain, front view.
PDB entry 1qba coloured by chain, side view.
PDB entry 1qba coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease.
Tews I, Perrakis A, Oppenheim A, Dauter Z, Wilson KS, Vorgias CE
Nat Struct Biol 3 638-48 (1996)
PMID: 8673609

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitobiase Chain: A
Molecule details ›
Chain: A
Length: 858 amino acids
Theoretical weight: 95.92 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54468 (Residues: 28-885; Coverage: 100%)
Gene name: chb
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 110.7Å b: 99.9Å c: 87.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.139 0.196
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Structural and sequence-based classification of glycoside hydrolases.
Henrissat et al. (1997)
21 more

14 mentions without citation

Computationally designed libraries for rapid enzyme stabilization.
Wijma et al. (2014)
13 more