› 1qcg

X-ray diffraction
2.1Å resolution

LOW TEMPERATURE STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN

Released:
DOI: 10.2210/pdb1qcg/pdb
PDB entry 1qcg coloured by chain, front view.
PDB entry 1qcg coloured by chain, side view.
PDB entry 1qcg coloured by chain, top view.
Source organism: Phytolacca americana
Primary publication:
X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs.
Kurinov IV, Myers DE, Irvin JD, Uckun FM
Protein Sci 8 1765-72 (1999)
PMID: 10493577

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein I Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.34 KDa
Source organism: Phytolacca americana
UniProt:
  • Canonical: P10297 (Residues: 23-284; Coverage: 90%)
Gene name: PAP1
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 48.68Å b: 49.09Å c: 64.56Å
α: 67.95° β: 82.9° γ: 65.37°
R-values:
R R work R free
0.208 0.191 0.27

Quick links

Citations

4 review citations

Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance.
Di et al. (2015)
3 more

1 mention without citation

Strategies to Improve the Clinical Utility of Saporin-Based Targeted Toxins.
Giansanti et al. (2018)