› 1qco

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF FUMARYLACETOACETATE HYDROLASE COMPLEXED WITH FUMARATE AND ACETOACETATE

Released:
DOI: 10.2210/pdb1qco/pdb
PDB entry 1qco coloured by chain, front view.
PDB entry 1qco coloured by chain, side view.
PDB entry 1qco coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure and mechanism of a carbon-carbon bond hydrolase.
Timm DE, Mueller HA, Bhanumoorthy P, Harp JM, Bunick GJ
Structure 7 1023-33 (1999)
PMID: 10508789

Function and Biology Details

Reaction catalysed: 
4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarylacetoacetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 46.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P35505 (Residues: 1-419; Coverage: 100%)
Gene name: Fah
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand NI 5 x NI
Ligand FUM 2 x FUM
Ligand AAE 2 x AAE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 64.64Å b: 110.94Å c: 67.81Å
α: 90° β: 102.52° γ: 90°
R-values:
R R work R free
0.159 0.159 0.192
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage.
Grogan G. (2005)
6 more

3 mentions without citation

Silent Tyrosinemia Type I Without Elevated Tyrosine or Succinylacetone Associated with Liver Cirrhosis and Hepatocellular Carcinoma.
Blackburn et al. (2016)
2 more