› 1qez

X-ray diffraction
2.7Å resolution

SULFOLOBUS ACIDOCALDARIUS INORGANIC PYROPHOSPHATASE: AN ARCHAEL PYROPHOSPHATASE.

Released:
DOI: 10.2210/pdb1qez/pdb
PDB entry 1qez coloured by chain, front view.
PDB entry 1qez coloured by chain, side view.
PDB entry 1qez coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
Sulfolobus acidocaldarius inorganic pyrophosphatase: structure, thermostability, and effect of metal ion in an archael pyrophosphatase.
Leppänen VM, Nummelin H, Hansen T, Lahti R, Schäfer G, Goldman A
Protein Sci 8 1218-31 (1999)
PMID: 10386872

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 173 amino acids
Theoretical weight: 19.4 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50308 (Residues: 1-173; Coverage: 100%)
Gene names: Saci_0955, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 70.12Å b: 98.73Å c: 107.25Å
α: 90° β: 78.01° γ: 90°
R-values:
R R work R free
0.197 0.197 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Bioenergetics of the Archaea.
Schäfer et al. (1999)
9 other articles

3 mentions without citation

Archaeal Inorganic Pyrophosphatase Displays Robust Activity under High-Salt Conditions and in Organic Solvents.
McMillan et al. (2016)
2 more