› 1qf9

X-ray diffraction
1.7Å resolution

PH INFLUENCES FLUORIDE COORDINATION NUMBER OF THE ALFX PHOSPHORYL TRANSFER TRANSITION STATE ANALOG IN UMP/CMP KINASE

Released:
DOI: 10.2210/pdb1qf9/pdb
PDB entry 1qf9 coloured by chain, front view.
PDB entry 1qf9 coloured by chain, side view.
PDB entry 1qf9 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
pH influences fluoride coordination number of the AlFx phosphoryl transfer transition state analog.
Schlichting I, Reinstein J
Nat Struct Biol 6 721-3 (1999)
PMID: 10426946

Function and Biology Details

Reaction catalysed: 
ATP + (d)CMP = ADP + (d)CDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UMP-CMP kinase Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.97 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P20425 (Residues: 2-195; Coverage: 100%)
Gene names: DDB_G0287495, ctpS, pyrK
Sequence domains: Adenylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ALF 1 x ALF
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand C5P 1 x C5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 78Å b: 78Å c: 100.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.244
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The biochemistry and physiology of metallic fluoride: action, mechanism, and implications.
Li L. (2003)
5 more

2 mentions without citation

A comprehensive update of the sequence and structure classification of kinases.
Cheek et al. (2005)
1 more