› 1qg8

X-ray diffraction
1.5Å resolution

NATIVE (MAGNESIUM-CONTAINING) SPSA FROM BACILLUS SUBTILIS

Released:
DOI: 10.2210/pdb1qg8/pdb
PDB entry 1qg8 coloured by chain, front view.
PDB entry 1qg8 coloured by chain, side view.
PDB entry 1qg8 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms.
Charnock SJ, Davies GJ
Biochemistry 38 6380-5 (1999)
PMID: 10350455

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spore coat polysaccharide biosynthesis protein SpsA Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 30.09 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39621 (Residues: 2-256; Coverage: 100%)
Gene names: BSU37910, ipa-63d, spsA
Sequence domains: Glycosyl transferase family 2
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2221
Unit cell:
a: 47.42Å b: 141.997Å c: 81.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.21
Expression system: Escherichia coli BL21

Quick links

Citations

49 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
48 more

4 mentions without citation

Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.
Araujo-Garrido et al. (2020)
3 more