› 1qgi

X-ray diffraction
1.6Å resolution

CHITOSANASE FROM BACILLUS CIRCULANS

Released:
DOI: 10.2210/pdb1qgi/pdb
PDB entry 1qgi coloured by chain, front view.
PDB entry 1qgi coloured by chain, side view.
PDB entry 1qgi coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism.
Saito J, Kita A, Higuchi Y, Nagata Y, Ando A, Miki K
J Biol Chem 274 30818-25 (1999)
PMID: 10521473

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitosanase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.99 KDa
Source organism: Niallia circulans
Expression system: Brevibacillus brevis
UniProt:
  • Canonical: P33673 (Residues: 43-200, 202-301; Coverage: 100%)
Gene name: csn
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GCS
Carbohydrate polymer
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21212
Unit cell:
a: 43.3Å b: 128Å c: 57.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.235
Expression system: Brevibacillus brevis

Quick links

Citations

7 review citations

Chitosan and its antimicrobial potential--a critical literature survey.
Raafat et al. (2009)
6 more

5 mentions without citation

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.
Viens et al. (2015)
4 more