› 1qh6

X-ray diffraction
2Å resolution

CATALYSIS AND SPECIFICITY IN ENZYMATIC GLYCOSIDE HYDROLASES: A 2,5B CONFORMATION FOR THE GLYCOSYL-ENZYME INTERMIDIATE REVEALED BY THE STRUCTURE OF THE BACILLUS AGARADHAERENS FAMILY 11 XYLANASE

Released:
DOI: 10.2210/pdb1qh6/pdb
PDB entry 1qh6 coloured by chain, front view.
PDB entry 1qh6 coloured by chain, side view.
PDB entry 1qh6 coloured by chain, top view.
Source organism: Salipaludibacillus agaradhaerens
Primary publication:
Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase.
Sabini E, Sulzenbacher G, Dauter M, Dauter Z, Jørgensen PL, Schülein M, Dupont C, Davies GJ, Wilson KS
Chem Biol 6 483-92 (1999)
PMID: 10381409

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.16 KDa
Source organism: Salipaludibacillus agaradhaerens
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q7SIE3 (Residues: 1-207; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: X2F, XYP
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 71.91Å b: 74.83Å c: 78.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.142 0.189
Expression system: Bacillus subtilis

Quick links

Citations

5 review citations

Glycosidase mechanisms.
Rye et al. (2000)
4 more

3 mentions without citation

X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
Unligil et al. (2000)
2 more