› 1qhh

X-ray diffraction
2.5Å resolution

STRUCTURE OF DNA HELICASE WITH ADPNP

Released:
DOI: 10.2210/pdb1qhh/pdb
PDB entry 1qhh coloured by chain, front view.
PDB entry 1qhh coloured by chain, side view.
PDB entry 1qhh coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase.
Soultanas P, Dillingham MS, Velankar SS, Wigley DB
J Mol Biol 290 137-48 (1999)
PMID: 10388562

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157446 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP-dependent DNA helicase PcrA Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.85 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 1-167; Coverage: 23%)
Gene name: pcrA
Sequence domains: UvrD/REP helicase N-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
ATP-dependent DNA helicase PcrA Chain: B
Molecule details ›
Chain: B
Length: 273 amino acids
Theoretical weight: 32.42 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 168-440; Coverage: 38%)
Gene name: pcrA
Sequence domains:
ATP-dependent DNA helicase PcrA Chain: C
Molecule details ›
Chain: C
Length: 115 amino acids
Theoretical weight: 12.82 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 441-555; Coverage: 16%)
Gene name: pcrA
Sequence domains: UvrD-like helicase C-terminal domain
Structure domains: PCRA; domain 4
ATP-dependent DNA helicase PcrA Chain: D
Molecule details ›
Chain: D
Length: 169 amino acids
Theoretical weight: 18.56 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 556-724; Coverage: 23%)
Gene name: pcrA
Sequence domains: PcrA/UvrD tudor domain
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS
Spacegroup: P65
Unit cell:
a: 138.936Å b: 138.936Å c: 111.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.285
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
11 more

5 mentions without citation

Viral and cellular RNA helicases as antiviral targets.
Kwong et al. (2005)
4 more