› 1qht

X-ray diffraction
2.1Å resolution

DNA POLYMERASE FROM THERMOCOCCUS SP. 9ON-7 ARCHAEON

Released:
DOI: 10.2210/pdb1qht/pdb
PDB entry 1qht coloured by chain, front view.
PDB entry 1qht coloured by chain, side view.
PDB entry 1qht coloured by chain, top view.
Source organism: Thermococcus sp. 9oN-7
Primary publication:
Crystal structure of a pol alpha family DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. 9 degrees N-7.
Rodriguez AC, Park HW, Mao C, Beese LS
J Mol Biol 299 447-62 (2000)
PMID: 10860752

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 775 amino acids
Theoretical weight: 89.83 KDa
Source organism: Thermococcus sp. 9oN-7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56366 (Residues: 1-775; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 95.3Å b: 98.7Å c: 112.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.312
Expression system: Escherichia coli

Quick links

Citations

13 review citations

DNA polymerase gamma, the mitochondrial replicase.
Kaguni LS. (2004)
12 more

3 mentions without citation

Structural insight into translesion synthesis by DNA Pol II.
Wang et al. (2009)
2 more