› 1qj8

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1qj8/pdb
PDB entry 1qj8 coloured by chain, front view.
PDB entry 1qj8 coloured by chain, side view.
PDB entry 1qj8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence.
Vogt J, Schulz GE
Structure 7 1301-9 (1999)
PMID: 10545325

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein X Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A917 (Residues: 24-171; Coverage: 100%)
Gene names: JW0799, b0814, ompX, ybiG
Sequence domains: Outer membrane protein beta-barrel domain
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand C8E 1 x C8E
Ligand PCL 2 x PCL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: R32
Unit cell:
a: 82.4Å b: 82.4Å c: 206.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Structure and function of bacterial outer membrane proteins: barrels in a nutshell.
Koebnik et al. (2000)
19 more

55 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
54 more