› 1qkr

X-ray diffraction
1.8Å resolution

Crystal structure of the vinculin tail and a pathway for activation

Released:
DOI: 10.2210/pdb1qkr/pdb
PDB entry 1qkr coloured by chain, front view.
PDB entry 1qkr coloured by chain, side view.
PDB entry 1qkr coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystal structure of the vinculin tail suggests a pathway for activation.
Bakolitsa C, de Pereda JM, Bagshaw CR, Critchley DR, Liddington RC
Cell 99 603-13 (1999)
PMID: 10612396

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vinculin Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.63 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12003 (Residues: 947-1135; Coverage: 16%)
Gene names: VCL, VINC1
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 39.6Å b: 87.77Å c: 50.63Å
α: 90° β: 91.27° γ: 90°
R-values:
R R work R free
0.2 0.2 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

Paxillin: adapting to change.
Brown et al. (2004)
31 more

13 mentions without citation

GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin.
Schmalzigaug et al. (2007)
12 more