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X-ray diffraction
1.95Å resolution

Fab fragment (MN14C11.6) in complex with a peptide antigen derived from Neisseria meningitidis P1.7 serosubtype antigen and domain II from Streptococcal protein G

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210771 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.01 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 367-430; Coverage: 11%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)
ANTIBODY Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.95 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Major outer membrane protein P.IA Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 889 Da
Source organism: Neisseria meningitidis
Expression system: Not provided
UniProt:
  • Canonical: P57041 (Residues: 46-55; Coverage: 3%)
Gene names: NMA1642, porA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P21
Unit cell:
a: 43.482Å b: 63.099Å c: 89.374Å
α: 90° β: 81.54° γ: 90°
R-values:
R R work R free
0.209 0.205 0.262
Expression systems:
  • Escherichia coli
  • Not provided