› 1qkz

X-ray diffraction
1.95Å resolution

Fab fragment (MN14C11.6) in complex with a peptide antigen derived from Neisseria meningitidis P1.7 serosubtype antigen and domain II from Streptococcal protein G

Released:
DOI: 10.2210/pdb1qkz/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1qkz coloured by chain, front view.
PDB entry 1qkz coloured by chain, side view.
PDB entry 1qkz coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of an Fab fragment in complex with a meningococcal serosubtype antigen and a protein G domain.
Derrick JP, Maiden MC, Feavers IM
J Mol Biol 293 81-91 (1999)
PMID: 10512717

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210771 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.01 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 367-430; Coverage: 11%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)
ANTIBODY Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY Chain: L
Major outer membrane protein P.IA Chain: P

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P21
Unit cell:
a: 43.482Å b: 63.099Å c: 89.374Å
α: 90° β: 81.54° γ: 90°
R-values:
R R work R free
0.209 0.205 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Site-directed antibody immobilization techniques for immunosensors.
Makaraviciute et al. (2013)
16 other articles

11 mentions without citation

Fc-Binding Ligands of Immunoglobulin G: An Overview of High Affinity Proteins and Peptides.
Choe et al. (2016)
10 more