1qle

X-ray diffraction
3Å resolution

CRYO-STRUCTURE OF THE PARACOCCUS DENITRIFICANS FOUR-SUBUNIT CYTOCHROME C OXIDASE IN THE COMPLETELY OXIDIZED STATE COMPLEXED WITH AN ANTIBODY FV FRAGMENT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209274 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Cytochrome c oxidase subunit 1-beta Chain: A
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 60.35 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P98002 (Residues: 17-554; Coverage: 96%)
Gene name: ctaDII
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Structure domains: Cytochrome c oxidase-like, subunit I domain
Cytochrome c oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 27.97 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P08306 (Residues: 30-281; Coverage: 94%)
Gene names: coiI, ctaB, ctaC
Sequence domains:
Structure domains:
Cytochrome c oxidase subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 273 amino acids
Theoretical weight: 30.68 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P06030 (Residues: 2-274; Coverage: 100%)
Gene names: coiII, ctaE
Sequence domains: Cytochrome c oxidase subunit III
Structure domains:
Cytochrome c oxidase subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 43 amino acids
Theoretical weight: 4.7 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P77921 (Residues: 8-50; Coverage: 86%)
Gene name: ctaH
Sequence domains: Bacterial aa3 type cytochrome c oxidase subunit IV
Structure domains: Bacterial aa3 type cytochrome c oxidase subunit IV
HEAVY CHAIN ANTIBODY FV FRAGMENT Chain: H
Molecule details ›
Chain: H
Length: 119 amino acids
Theoretical weight: 13.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
LIGHT CHAIN ANTIBODY FV FRAGMENT Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P4
Unit cell:
a: 205.2Å b: 205.2Å c: 81.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.309
Expression system: Escherichia coli