› 1qlm

X-ray diffraction
2Å resolution

The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri

Released:
DOI: 10.2210/pdb1qlm/pdb
PDB entry 1qlm coloured by chain, front view.
PDB entry 1qlm coloured by chain, side view.
PDB entry 1qlm coloured by chain, top view.
Source organism: Methanopyrus kandleri
Primary publication:
The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri.
Grabarse W, Vaupel M, Vorholt JA, Shima S, Thauer RK, Wittershagen A, Bourenkov G, Bartunik HD, Ermler U
Structure 7 1257-68 (1999)
PMID: 10545331

Function and Biology Details

Reaction catalysed: 
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H(2)O = 5-formyl-5,6,7,8-tetrahydromethanopterin
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methenyltetrahydromethanopterin cyclohydrolase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.09 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli
UniProt:
  • Canonical: P94954 (Residues: 1-316; Coverage: 100%)
Gene names: MK0625, mch
Sequence domains: Cyclohydrolase (MCH)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P6322
Unit cell:
a: 125.9Å b: 125.9Å c: 172.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.219
Expression system: Escherichia coli

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Citations

1 review citation

Beating the acetyl coenzyme A-pathway to the origin of life.
Nitschke et al. (2013)
18 other articles

2 mentions without citation

Using neural networks and evolutionary information in decoy discrimination for protein tertiary structure prediction.
Tan et al. (2008)
1 more