› 1qln

X-ray diffraction
2.4Å resolution

STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX

Released:
DOI: 10.2210/pdb1qln/pdb
PDB entry 1qln coloured by chain, front view.
PDB entry 1qln coloured by chain, side view.
PDB entry 1qln coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structure of a transcribing T7 RNA polymerase initiation complex.
Cheetham GM, Steitz TA
Science 286 2305-9 (1999)
PMID: 10600732

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114439 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
DNA (5-D(P*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*TP*A)-3) Chain: N
Molecule details ›
Chain: N
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
DNA (5- D (P*CP*TP*CP*CP*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP* AP*TP*TP*A)-3) Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.72 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
RNA (5- R(PPP*GP*GP*G)-3) Chain: R
Molecule details ›
Chain: R
Length: 3 nucleotides
Theoretical weight: 1.15 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 221.2Å b: 73.6Å c: 80.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.267
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

24 review citations

Structural insights into translational fidelity.
Ogle et al. (2005)
23 more

46 mentions without citation

Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.
Basu et al. (2020)
45 more