› 1qlq

X-ray diffraction
1.42Å resolution

Bovine Pancreatic Trypsin Inhibitor (BPTI) Mutant with Altered Binding Loop Sequence

Released:
DOI: 10.2210/pdb1qlq/pdb
PDB entry 1qlq coloured by chain, front view.
PDB entry 1qlq coloured by chain, side view.
PDB entry 1qlq coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
High-resolution structure of bovine pancreatic trypsin inhibitor with altered binding loop sequence.
Czapinska H, Otlewski J, Krzywda S, Sheldrick GM, Jaskólski M
J Mol Biol 295 1237-49 (2000)
PMID: 10653700

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic trypsin inhibitor Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS SRA2
Spacegroup: P43212
Unit cell:
a: 52.71Å b: 52.71Å c: 43.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.11 not available 0.161
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure and function of invertebrate Kunitz serine protease inhibitors.
Ranasinghe et al. (2013)
1 more

4 mentions without citation

Identification of the ligand binding sites on the molecular surface of proteins.
Kinoshita et al. (2005)
3 more