› 1qmp

X-ray diffraction
2Å resolution

Phosphorylated aspartate in the crystal structure of the sporulation response regulator, Spo0A

Released:
DOI: 10.2210/pdb1qmp/pdb
PDB entry 1qmp coloured by chain, front view.
PDB entry 1qmp coloured by chain, side view.
PDB entry 1qmp coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Phosphorylated aspartate in the structure of a response regulator protein.
Lewis RJ, Brannigan JA, Muchová K, Barák I, Wilkinson AJ
J Mol Biol 294 9-15 (1999)
PMID: 10556024

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stage 0 sporulation protein A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 130 amino acids
Theoretical weight: 14.83 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52934 (Residues: 1-130; Coverage: 50%)
Gene name: spo0A
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand PHD 4 x PHD

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 69.197Å b: 72.859Å c: 114.297Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.195 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Two-component signal transduction.
Stock et al. (2000)
11 more

5 mentions without citation

Conformational changes in protein loops and helices induced by post-translational phosphorylation.
Groban et al. (2006)
4 more