› 1qmq

X-ray diffraction
1.55Å resolution

Optical detection of cytochrome P450 by sensitizer-linked substrates

Released:
DOI: 10.2210/pdb1qmq/pdb
PDB entry 1qmq coloured by chain, front view.
PDB entry 1qmq coloured by chain, side view.
PDB entry 1qmq coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Optical detection of cytochrome P450 by sensitizer-linked substrates.
Dmochowski IJ, Crane BR, Wilker JJ, Winkler JR, Gray HB
Proc Natl Acad Sci U S A 96 12987-90 (1999)
PMID: 10557259

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.56 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand DRB 2 x DRB
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 65.39Å b: 74.47Å c: 91.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.226
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Targeting proteins with metal complexes.
Meggers E. (2009)
4 more

7 mentions without citation

MOLE 2.0: advanced approach for analysis of biomacromolecular channels.
Sehnal et al. (2013)
6 more