› 1qqc

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF AN ARCHAEBACTERIAL DNA POLYMERASE D.TOK

Released:
DOI: 10.2210/pdb1qqc/pdb
PDB entry 1qqc coloured by chain, front view.
PDB entry 1qqc coloured by chain, side view.
PDB entry 1qqc coloured by chain, top view.
Source organism: Desulfurococcus sp. Tok
Primary publication:
Crystal structure of an archaebacterial DNA polymerase.
Zhao Y, Jeruzalmi D, Moarefi I, Leighton L, Lasken R, Kuriyan J
Structure 7 1189-99 (1999)
PMID: 10545321

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 773 amino acids
Theoretical weight: 90.13 KDa
Source organism: Desulfurococcus sp. Tok
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SIG7 (Residues: 1-773; Coverage: 100%)
Gene name: pol
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 66.1Å b: 107.6Å c: 155.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.296 0.244 0.296
Expression system: Escherichia coli

Quick links

Citations

7 review citations

DNA polymerases that propagate the eukaryotic DNA replication fork.
Garg et al. (2005)
6 more

2 mentions without citation

DNA polymerase epsilon: a polymerase of unusual size (and complexity).
Pursell et al. (2008)
1 more