› 1qqi

Solution NMR

SOLUTION STRUCTURE OF THE DNA-BINDING AND TRANSACTIVATION DOMAIN OF PHOB FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1qqi/pdb
PDB entry 1qqi coloured by chain, front view.
PDB entry 1qqi coloured by chain, side view.
PDB entry 1qqi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box.
Okamura H, Hanaoka S, Nagadoi A, Makino K, Nishimura Y
J Mol Biol 295 1225-36 (2000)
PMID: 10653699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate regulon transcriptional regulatory protein PhoB Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFJ5 (Residues: 126-229; Coverage: 45%)
Gene names: JW0389, b0399, phoB
Sequence domains: Transcriptional regulatory protein, C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: 4D SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Prokaryotic transcription regulators: more than just the helix-turn-helix motif.
Huffman et al. (2002)
3 more

7 mentions without citation

DISPLAR: an accurate method for predicting DNA-binding sites on protein surfaces.
Tjong et al. (2007)
6 more