› 1qr6

X-ray diffraction
2.1Å resolution

HUMAN MITOCHONDRIAL NAD(P)-DEPENDENT MALIC ENZYME

Released:
DOI: 10.2210/pdb1qr6/pdb
PDB entry 1qr6 coloured by chain, front view.
PDB entry 1qr6 coloured by chain, side view.
PDB entry 1qr6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human mitochondrial NAD(P)(+)-dependent malic enzyme: a new class of oxidative decarboxylases.
Xu Y, Bhargava G, Wu H, Loeber G, Tong L
Structure 7 877-889 (1999)
PMID: 10467136

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 66.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23368 (Residues: 1-584; Coverage: 100%)
Gene name: ME2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2, NSLS BEAMLINE X4A
Unit cell:
a: 204.4Å b: 107Å c: 59.2Å
α: 90° β: 90° γ: 101.9°
R-values:
R R work R free
0.228 0.228 0.287
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Advances in multiple wavelength anomalous diffraction crystallography.
Ealick SE. (2000)
3 more

9 mentions without citation

Xyloketal B, a marine compound, acts on a network of molecular proteins and regulates the activity and expression of rat cytochrome P450 3a: a bioinformatic and animal study.
Su et al. (2014)
8 more