› 1qsl

X-ray diffraction
2.2Å resolution

KLENOW FRAGMENT COMPLEXED WITH SINGLE-STRANDED SUBSTRATE AND EUROPIUM (III) ION

Released:
DOI: 10.2210/pdb1qsl/pdb
PDB entry 1qsl coloured by chain, front view.
PDB entry 1qsl coloured by chain, side view.
PDB entry 1qsl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural elucidation of the binding and inhibitory properties of lanthanide (III) ions at the 3'-5' exonucleolytic active site of the Klenow fragment.
Brautigam CA, Aschheim K, Steitz TA
Chem Biol 6 901-8 (1999)
PMID: 10631518

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114468 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00582 (Residues: 324-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains:
Structure domains:
5'-D(*GP*CP*TP*TP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EU 1 x EU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43
Unit cell:
a: 103.3Å b: 103.3Å c: 86.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Structural characteristics of protein binding sites for calcium and lanthanide ions.
Pidcock et al. (2001)
2 more

3 mentions without citation

The spectrum of WRN mutations in Werner syndrome patients.
Huang et al. (2006)
2 more