› 1qto

X-ray diffraction
1.5Å resolution

1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS

Released:
DOI: 10.2210/pdb1qto/pdb
PDB entry 1qto coloured by chain, front view.
PDB entry 1qto coloured by chain, side view.
PDB entry 1qto coloured by chain, top view.
Source organism: Streptomyces verticillus
Primary publication:
The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus.
Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M
J Mol Biol 295 915-25 (2000)
PMID: 10656800

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin resistance protein Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.24 KDa
Source organism: Streptomyces verticillus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53793 (Residues: 1-122; Coverage: 100%)
Gene name: ble
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P21212
Unit cell:
a: 54.94Å b: 67.88Å c: 35.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.221
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
1 more

10 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
9 more