› 1qvc

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI

Released:
DOI: 10.2210/pdb1qvc/pdb
PDB entry 1qvc coloured by chain, front view.
PDB entry 1qvc coloured by chain, side view.
PDB entry 1qvc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis.
Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N
J Biochem 127 329-35 (2000)
PMID: 10731701

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 145 amino acids
Theoretical weight: 15.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGE0 (Residues: 2-146; Coverage: 82%)
Gene names: JW4020, b4059, exrB, lexC, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3, PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 105.369Å b: 62.923Å c: 97.792Å
α: 90° β: 112.57° γ: 90°
R-values:
R R work R free
0.247 0.247 0.317
Expression system: Escherichia coli

Quick links

Citations

8 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
7 more

19 mentions without citation

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
18 more