› 1qwo

X-ray diffraction
1.5Å resolution

Crystal structure of a phosphorylated phytase from Aspergillus fumigatus, revealing the structural basis for its heat resilience and catalytic pathway

Released:
DOI: 10.2210/pdb1qwo/pdb
PDB entry 1qwo coloured by chain, front view.
PDB entry 1qwo coloured by chain, side view.
PDB entry 1qwo coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Crystal structure of a heat-resilient phytase from Aspergillus fumigatus, carrying a phosphorylated histidine.
Xiang T, Liu Q, Deacon AM, Koshy M, Kriksunov IA, Lei XG, Hao Q, Thiel DJ
J Mol Biol 339 437-45 (2004)
PMID: 15136045

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase A Chain: A
Molecule details ›
Chain: A
Length: 442 amino acids
Theoretical weight: 48.62 KDa
Source organism: Aspergillus fumigatus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O00092 (Residues: 24-465; Coverage: 100%)
Gene names: AFUA_4G08630, phyA, phyA3
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
1 modified residue:
Ligand NEP 1 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 70.34Å b: 70.34Å c: 186.673Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.186
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Phytate: impact on environment and human nutrition. A challenge for molecular breeding.
Bohn et al. (2008)
3 more

5 mentions without citation

Crystallographic refinement of ligand complexes.
Kleywegt GJ. (2007)
4 more