› 1qx0

X-ray diffraction
2.26Å resolution

CONJUGATIVE RELAXASE TRWC IN COMPLEX WITH ORIT DNA. METAL-BOUND STRUCTURE

Released:
DOI: 10.2210/pdb1qx0/pdb
PDB entry 1qx0 coloured by chain, front view.
PDB entry 1qx0 coloured by chain, side view.
PDB entry 1qx0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC.
Guasch A, Lucas M, Moncalián G, Cabezas M, Pérez-Luque R, Gomis-Rüth FX, de la Cruz F, Coll M
Nat Struct Biol 10 1002-10 (2003)
PMID: 14625590

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114476 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TrwC relaxase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47673 (Residues: 1-293; Coverage: 30%)
Gene name: trwC
Sequence domains: TrwC relaxase
DNA OLIGONUCLEOTIDE Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 7.71 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6122
Unit cell:
a: 91.185Å b: 91.185Å c: 205.034Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.234 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

Mobility of plasmids.
Smillie et al. (2010)
20 more

8 mentions without citation

The structure of the minimal relaxase domain of MobA at 2.1 A resolution.
Monzingo et al. (2007)
7 more