› 1qxh

X-ray diffraction
2.2Å resolution

Crystal Structure of Escherichia coli Thiol Peroxidase in the Oxidized State

Released:
DOI: 10.2210/pdb1qxh/pdb
PDB entry 1qxh coloured by chain, front view.
PDB entry 1qxh coloured by chain, side view.
PDB entry 1qxh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli thiol peroxidase in the oxidized state: insights into intramolecular disulfide formation and substrate binding in atypical 2-Cys peroxiredoxins.
Choi J, Choi S, Choi J, Cha MK, Kim IH, Shin W
J Biol Chem 278 49478-86 (2003)
PMID: 14506251

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 17.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A862 (Residues: 2-168; Coverage: 99%)
Gene names: JW1317, b1324, tpx, yzzJ
Sequence domains: Redoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P212121
Unit cell:
a: 38.654Å b: 58.428Å c: 127.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Why do bacteria use so many enzymes to scavenge hydrogen peroxide?
Mishra et al. (2012)
4 more

4 mentions without citation

Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins.
Hall et al. (2011)
3 more