› 1qzz

X-ray diffraction
2.1Å resolution

Crystal structure of aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM)

Released:
DOI: 10.2210/pdb1qzz/pdb
PDB entry 1qzz coloured by chain, front view.
PDB entry 1qzz coloured by chain, side view.
PDB entry 1qzz coloured by chain, top view.
Source organism: Streptomyces purpurascens
Primary publication:
Crystal structure of aclacinomycin-10-hydroxylase, a S-adenosyl-L-methionine-dependent methyltransferase homolog involved in anthracycline biosynthesis in Streptomyces purpurascens.
Jansson A, Niemi J, Lindqvist Y, Mäntsälä P, Schneider G
J Mol Biol 334 269-80 (2003)
PMID: 14607118

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aclacinomycin 10-hydroxylase RdmB Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 39.84 KDa
Source organism: Streptomyces purpurascens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54527 (Residues: 1-374; Coverage: 100%)
Gene name: rdmB
Sequence domains: O-methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11, EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2221
Unit cell:
a: 63.12Å b: 92.13Å c: 115.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.204 0.275
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
7 more

10 mentions without citation

Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.
Parsons et al. (2007)
9 more