› 1r0j

X-ray diffraction
2Å resolution

nickel-substituted rubredoxin

Released:
DOI: 10.2210/pdb1r0j/pdb
PDB entry 1r0j coloured by chain, front view.
PDB entry 1r0j coloured by chain, side view.
PDB entry 1r0j coloured by chain, top view.
Source organism: Clostridium pasteurianum
Primary publication:
Metal-substituted derivatives of the rubredoxin from Clostridium pasteurianum.
Maher M, Cross M, Wilce MC, Guss JM, Wedd AG
Acta Crystallogr D Biol Crystallogr 60 298-303 (2004)
PMID: 14747706

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.05 KDa
Source organism: Clostridium pasteurianum
Expression system: Escherichia coli
UniProt:
  • Canonical: P00268 (Residues: 1-54; Coverage: 100%)
Sequence domains: Rubredoxin
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 64.331Å b: 64.331Å c: 32.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.219 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Iron-sulfur protein folds, iron-sulfur chemistry, and evolution.
Meyer J. (2008)
12 other articles

2 mentions without citation

Experimental and DFT Investigations Reveal the Influence of the Outer Coordination Sphere on the Vibrational Spectra of Nickel-Substituted Rubredoxin, a Model Hydrogenase Enzyme.
Slater et al. (2017)
1 more