› 1r15

X-ray diffraction
2.4Å resolution

Aplysia ADP ribosyl cyclase with bound nicotinamide and R5P

Released:
DOI: 10.2210/pdb1r15/pdb
PDB entry 1r15 coloured by chain, front view.
PDB entry 1r15 coloured by chain, side view.
PDB entry 1r15 coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate.
Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q
Structure 12 477-86 (2004)
PMID: 15016363

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 258 amino acids
Theoretical weight: 29.58 KDa
Source organism: Aplysia californica
Expression system: Escherichia coli
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand N 8 x N
Ligand NCA 16 x NCA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 60.4Å b: 75.32Å c: 138.13Å
α: 88.16° β: 89.22° γ: 89.09°
R-values:
R R work R free
0.245 0.244 0.269
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nicotinamide adenine dinucleotide: beyond a redox coenzyme.
Lin H. (2007)
1 more

3 mentions without citation

Mechanism of cyclizing NAD to cyclic ADP-ribose by ADP-ribosyl cyclase and CD38.
Graeff et al. (2009)
2 more