› 1r31

X-ray diffraction
2.1Å resolution

HMG-CoA reductase from Pseudomonas mevalonii complexed with HMG-CoA

Released:
DOI: 10.2210/pdb1r31/pdb
PDB entry 1r31 coloured by chain, front view.
PDB entry 1r31 coloured by chain, side view.
PDB entry 1r31 coloured by chain, top view.
Source organism: Pseudomonas mevalonii
Entry authors: Watson JM, Steussy CN, Burgner JW, Lawrence CM, Tabernero L, Rodwell VW, Stauffacher CV

Function and Biology Details

Reaction catalysed: 
(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxy-3-methylglutaryl-coenzyme A reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 45.64 KDa
Source organism: Pseudomonas mevalonii
Expression system: Escherichia coli
UniProt:
  • Canonical: P13702 (Residues: 1-428; Coverage: 100%)
Gene name: mvaA
Sequence domains: Hydroxymethylglutaryl-coenzyme A reductase
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 1 x COA
2 bound ligands:
Ligand SO4 2 x SO4
Ligand MEV 2 x MEV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: I4132
Unit cell:
a: 227.369Å b: 227.369Å c: 227.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.241
Expression system: Escherichia coli

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