› 1r3m

X-ray diffraction
2.2Å resolution

Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease

Released:
DOI: 10.2210/pdb1r3m/pdb
PDB entry 1r3m coloured by chain, front view.
PDB entry 1r3m coloured by chain, side view.
PDB entry 1r3m coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease.
Berisio R, Sica F, De Lorenzo C, Di Fiore A, Piccoli R, Zagari A, Mazzarella L
FEBS Lett 554 105-10 (2003)
PMID: 14596923

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.63 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 49.48Å b: 57.55Å c: 53.06Å
α: 90° β: 116° γ: 90°
R-values:
R R work R free
0.223 0.211 0.292

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Citations

2 review citations

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
1 more

2 mentions without citation

Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity.
Rutkoski et al. (2008)
1 more