› 1r5j

X-ray diffraction
2.7Å resolution

Crystal Structure of a Phosphotransacetylase from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb1r5j/pdb
PDB entry 1r5j coloured by chain, front view.
PDB entry 1r5j coloured by chain, side view.
PDB entry 1r5j coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Crystal structure of a phosphotransacetylase from Streptococcus pyogenes.
Xu QS, Shin DH, Pufan R, Yokota H, Kim R, Kim SH
Proteins 55 479-81 (2004)
PMID: 15048838

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 36.67 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZQ5 (Residues: 1-331; Coverage: 100%)
Gene names: SPy_1128, pta
Sequence domains: Phosphate acetyl/butaryl transferase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4332
Unit cell:
a: 173.725Å b: 173.725Å c: 173.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.281
Expression system: Escherichia coli

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Citations

1 review citation

Structure and function of enzymes involved in the anaerobic degradation of L-threonine to propionate.
Simanshu et al. (2007)
7 other articles