› 1r61

X-ray diffraction
2.5Å resolution

The structure of predicted metal-dependent hydrolase from Bacillus stearothermophilus

Released:
DOI: 10.2210/pdb1r61/pdb
PDB entry 1r61 coloured by chain, front view.
PDB entry 1r61 coloured by chain, side view.
PDB entry 1r61 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Maderova J, Borek D, Tomchick D, Joachimiak A, Collart F, Otwinowski Z, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-160830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 23.08 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84132 (Residues: 1-207; Coverage: 100%)
Sequence domains: Putative cyclase
Structure domains: Putative cyclase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM, APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 118.434Å b: 118.434Å c: 123.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli

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