› 1r6z

X-ray diffraction
2.8Å resolution

The Crystal Structure of the Argonaute2 PAZ domain (as a MBP fusion)

Released:
DOI: 10.2210/pdb1r6z/pdb
PDB entry 1r6z coloured by chain, front view.
PDB entry 1r6z coloured by chain, side view.
PDB entry 1r6z coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes.
Song JJ, Liu J, Tolia NH, Schneiderman J, Smith SK, Martienssen RA, Hannon GJ, Joshua-Tor L
Nat Struct Biol 10 1026-32 (2003)
PMID: 14625589

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Protein argonaute-2 Chains: A, P, Z
Molecule details ›
Chains: A, P, Z
Length: 509 amino acids
Theoretical weight: 56.47 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9VUQ5 (Residues: 591-726; Coverage: 11%)
Gene names: AGO2, CG7439, JW3994, b4034, malE
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand NI 9 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P65
Unit cell:
a: 89.811Å b: 89.811Å c: 380.326Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.227 0.281
Expression system: Escherichia coli

Quick links

Citations

115 review citations

MicroRNAs: genomics, biogenesis, mechanism, and function.
Bartel DP. (2004)
114 more

6 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
5 more