› 1r77

X-ray diffraction
1.75Å resolution

Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1

Released:
DOI: 10.2210/pdb1r77/pdb
PDB entry 1r77 coloured by chain, front view.
PDB entry 1r77 coloured by chain, side view.
PDB entry 1r77 coloured by chain, top view.
Source organism: Staphylococcus capitis
Primary publication:
Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges.
Lu JZ, Fujiwara T, Komatsuzawa H, Sugai M, Sakon J
J Biol Chem 281 549-58 (2006)
PMID: 16257954

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycyl-glycine endopeptidase ALE-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Staphylococcus capitis
Expression system: Escherichia coli
UniProt:
  • Canonical: O05156 (Residues: 271-362; Coverage: 28%)
Sequence domains: Bacterial SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 45.199Å b: 58.521Å c: 85.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.232
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Bacterial peptidoglycan (murein) hydrolases.
Vollmer et al. (2008)
14 more

15 mentions without citation

Combination therapy with lysin CF-301 and antibiotic is superior to antibiotic alone for treating methicillin-resistant Staphylococcus aureus-induced murine bacteremia.
Schuch et al. (2014)
14 more