› 1r7a

X-ray diffraction
1.77Å resolution

Sucrose Phosphorylase from Bifidobacterium adolescentis

Released:
DOI: 10.2210/pdb1r7a/pdb
PDB entry 1r7a coloured by chain, front view.
PDB entry 1r7a coloured by chain, side view.
PDB entry 1r7a coloured by chain, top view.
Source organism: Bifidobacterium adolescentis
Primary publication:
Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis.
Sprogøe D, van den Broek LA, Mirza O, Kastrup JS, Voragen AG, Gajhede M, Skov LK
Biochemistry 43 1156-62 (2004)
PMID: 14756551

Function and Biology Details

Reaction catalysed: 
Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sucrose phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 504 amino acids
Theoretical weight: 56.28 KDa
Source organism: Bifidobacterium adolescentis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0ZZH6 (Residues: 1-504; Coverage: 100%)
Gene names: BAD_0078, spl, sucP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, MAX II BEAMLINE I711, BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 55.04Å b: 123.36Å c: 151.481Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.195
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Understanding nature's catalytic toolkit.
Gutteridge et al. (2005)
11 more

10 mentions without citation

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering.
Franceus et al. (2020)
9 more